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来源：百度知道 编辑：UC知道 时间：2024/06/05 10:23:03
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本实验利用CTAB法提取了四角蛤蜊斧足的DNA，然后用琼脂糖凝胶对所提DNA进行电泳和紫外检测系统检测；选用提取较好的DNA样品作为PCR反应的模板，通过设计两条18SrRNA基因3′端引物，然后进行PCR扩增；PCR结束后，对所扩增的产物经琼脂糖凝胶电泳检测合格后送上海生工纯化，进行双向测序；所得序列用DNAStar软件组装，再进行校对。结果显示：四角蛤蜊18SrRNA基因3′端序列长度为629 bp，其A、T、G、C的含量分别为22.10%、26.55 %、24.80%、26.55 %，G+C含量为51.35%；与别的同学所做的片段进行拼接，再进行比对分析其亲源关系。

这样的 Experimental use of the CTAB extraction of 15.40 clams Hammer adequate DNA, Then on agarose gel electrophoresis of DNA detection systems and UV detection; Extraction better use DNA samples as PCR template, the reaction through the design of two 18S rRNA gene-3 'primers, and then PCR PCR after amplification on the expansion of the product by agarose gel electrophoresis detection qualified evacuation Shanghai Public Health purified - 377; Sequences from using DNA Star software assembly and then proofread. The results showed : 15.40 clams 18S rRNA gene 3 'sequence length of 629 bp, A, T, G, C content of 22.10%,26.55 %,24.80%,26.55% G + C content of 51.35%; with other students doing the fragments were spliced further than its analysis of the pro-source relationship.

Experimental use of the CTAB extraction of 15.40 clams Hammer adequate DNA, Then on agarose gel electrophoresis of DNA detection systems and UV detection; Extraction better use DNA samples as PCR template,