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A striking feature of these growth experiments
with glucose or acetate is that the total activity of
this enzyme continued to increase in cultures well
after the time at which the cells enter the stationary
phase. The greatest increase in enzyme
activity occurs after the cells have stopped
multiplying. In Fig. 3 are plotted the results of
two experiments, one with glucose and the other
acetate. The total enzyme activity of the crude
homogenates (containing mitochondria, microsomes,
and soluble supernatant fluid) is shown as
a function of the protein content of these extracts.
Since the experiments described above indicated
that the distribution of this enzyme changed
significantly in aerobic cultures after the cells
entered the stationary phase, standing cultures
were also incubated for prolonged periods to see
whether a similar phenomenon occurred. Washed
cells were fractionated and assayed daily

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