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This experiment (TG1) the genome team takes the template by the backwoods coli, the use molecular biology method, applies the PCR technology to clone the adenosine methionine synzyme from the backwoods coli code gene metK, after and connects the PCR recycling product the T cloning vehicle, transforms E.coli DH5a to do the gene bank, after connecting high expression vector PET28a, inducts E.coli in BL21(DE3) to carry on expresses massively. Again the expression vector through the construction fusion and the natural protein metK gene PCR to increase the product on 1% agarose gelatin the electrophoresis, the explanation recombination gene to be whether accurate; Through to constructs the fusion protein the cloning vehicle, the expression vector and uses in constructing the natural protein the cloning vehicle and the expression vector the double enzyme to cut the gel electrophoresis analysis, explains the goal gene accurate load; Through protein SDS-PAGE, indicated that the purification