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来源:百度知道 编辑:UC知道 时间:2024/05/29 05:16:25
1, Post-harvested grape berries on cold storage has two kinds of different gray mold symptoms: one sporulate on grape berries rapidly and develop a large amount of spores,cause the grape decay rapidly,the other develop no spores on grape berries,only mycelium.

5, Use species-specific primers to amplify B. cinerea species-specific fragment showed that only EXFG Ⅲ has this fragment with differences in sequence among strains, confirmed the morphological classification result that EXFG Ⅲ is typical B.cinerea. Further amplification of the 1171bp fragment of B. cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment, HhaI digestion show no difference in sequence. These results show that EXFG Ⅱ and EXFG Ⅲ may be two different subspecies in B.cinerea. EXFG Ⅰ has no B. cinerea specific sequence, and may be other species of the genus Botrytis.

6, Analysis the differences in rDNA-ITS sequence of 3 groups, the result showed that the rDNA-ITS sequence of 3 groups o

1, Post-harvested grape berries on cold storage manifest two kinds of different gray mold symptoms: the first sporulate on grape berries rapidly and develop a large amount of spores,cause the grape to decay rapidly, the second develop no spores on grape berries,only mycelium.

5, Using species-specific primers to amplify B. cinerea species-specific fragments showed that only EXFG Ⅲ displays this fragment with differences in sequence among strains, and confirms the morphological classification result that EXFG Ⅲ is a typical B.cinerea. Further amplification of the 1171bp fragment of B. cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment, while HhaI digestion shows no difference in sequence. These results show that EXFG Ⅱ and EXFG Ⅲ may be classifeid as two different subspecies of B.cinerea. EXFG Ⅰ has no B. cinerea specific sequence, and may be just another species of the genus Botrytis.

6, The results of the analysis of the differences in rDNA-ITS