请教一篇文章翻译〈Dynamic binding orientations direct activity of HIV reverse transcriptase〉

来源：百度知道 编辑：UC知道 时间：2024/06/02 00:19:28
前言是“The reverse transcriptase of human immunodeficiency virus (HIV) catalyses a series of reactions to convert the single-stranded RNA genome of HIV into double-stranded DNA for host-cell integration. ……However, the mechanism by which substrates regulate reverse transcriptase activities remains unclear.Here we report distinct orientational dynamics of reverse transcriptase observed on different substrates with a single-molecule assay. The enzyme adopted opposite binding orientations on duplexes containing DNA or RNA primers,directing its DNA synthesis or RNA hydrolysis activity,respectively. On duplexes containing the unique polypurine RNA primers for plus-strand DNA synthesis, the enzyme an rapidly switch between the two orientations. The switching kinetics were regulated by cognate nucleotides and non-nucleoside reverse transcriptase inhibitors, a major class of anti-HIV drugs. These results indicate that the activities of reverse transcriptase are determined by its binding orientati

该反转录酶的人类免疫缺陷病毒（ HIV ）的催化了一系列的反应，转换为单滞留的RNA基因组的艾滋病毒进入双滞留的DNA为东道国细胞融合。 … …但是，机制，使基板规范反转录酶的活动，仍然是我们unclear.here报告鲜明取向的动态反转录观察不同衬底上与单分子检测。酶通过具有约束力的方向相反，对复式含有DNA或RNA的引物，指示其DNA合成或RNA的水解活性，分别为。对复式载有独特的polypurine RNA的引物为加链DNA的合成，这种酶是一个快速开关两国之间的取向。开关动力学的规管同源核苷酸和非核苷类逆转录酶抑制剂，主要类抗艾滋病毒药物。这些结果表明，该活动的反转录酶是取决于其约束力的取向对基板“