医学论文摘要翻译

来源：百度知道 编辑：UC知道 时间：2024/06/22 17:53:54
摘要：目的 研究骨髓间充质干细胞在内毒素诱发急性肺损伤(ALI)兔模型中的保护作用。方法 实验于2007-10/2008-01在唐都医院中心实验室完成。成年健康雄性家兔20只，其中2只用于培养骨髓培养骨髓间充质干细胞（MSCs），余18只随机分为三组：内毒素(LPS)致ALI模型组(LPS组)、生理盐水对照组(NS组)、MSCs治疗组。LPS组、MSCs组利用内毒素气管内滴注法建立家兔急性肺损伤模型，模型建立成功后30min,MSCs组经家兔右侧颈静脉注射方式给予骨髓间充质干细胞，LPS组给与等量生理盐水，NS组按照以上两组造模、治疗方法均给予等量生理盐水处理，所有动物观察48小时后处死，细胞计数板计算右肺灌洗液中的总细胞、中性粒细胞数、BCA法计算总蛋白渗出量。左上肺石蜡包埋HE染色观察病理变化，左下肺称干湿重，计算干湿比。结果 镜下：LPS组肺泡腔和小气道内有大量炎性细胞浸润,明显出血水肿。肺泡间隔明显增厚，MSCs组肺泡壁较薄，只有少量的炎性细胞浸润；统计学上LPS模型组支气管肺泡灌洗液（BALF）中炎性细胞数目、肺组织干湿比以及蛋白渗出量较NS组有统计学意义（P<0.05）；MSCs组较NS组P>0.05，无统计学意义。结论 MSCs可能通过在损伤的家兔肺组织中转化为上皮细胞，从而降低支气管肺泡灌洗液中的炎性细胞、蛋白的渗出，对急性肺损伤具有一定的保护作用。
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Abstract: The purpose of the study of bone marrow mesenchymal stem cells, toxin-induced acute lung injury (ALI) rabbit model of protection. 2007-10/2008-01 experimental methods in all hospitals in the Tang Center for the completion of the laboratory. Healthy adult male rabbits, 20, of which 2 for the training culture of bone marrow mesenchymal stem cells (MSCs), more than 18 were randomly divided into three groups: endotoxin (LPS)-induced ALI model group (LPS), saline In the control group (NS group), MSCs in the treatment group. LPS group, MSCs group using tracheal instillation endotoxic established rabbit model of acute lung injury, set up after the success of the model 30min, MSCs group of rabbits by injecting the right side of the jugular vein to give bone marrow mesenchymal stem cells, LPS, and other groups to give Volume of normal saline, NS Group for more than two groups in accordance with the model, are given equal treatment to deal with normal saline, all animals to observe