冻干粉蛋白质试剂如何分装?

来源:百度知道 编辑:UC知道 时间:2024/06/16 12:48:40
我买的是sigma公司的血管紧张素Ⅱ冻干粉1mg,我每次用量很小,向分装以下,应该怎么办?
怎么样算是适量?我又不想称量损失试剂,因为只有1mg啊

楼主如果订的是Angiotensin II human 的话你可以看下sigma的datasheet
Storage/Stability
It is recommended to store angiotensin II at –20 C.
Lyophilized peptides are generally extraordinarily stable
at sub-zero temperatures, often showing little or no
degradation even after years. To avoid condensation of
moisture in the bottle, allow the vial to reach room
temperature before opening. If the contents of a vial
are not used immediately, blanket with an inert gas
(nitrogen) and store desiccated in the dark at –20 C.
Stock solutions at neutral pH stored at –20 C should
remain active for at least two months. Avoid repeated
freeze-thaw cycles. The reconstituted peptide solution
may also be aliquoted, relyophilized, and stored
at –20 C. Peptides in solution are also susceptible to
proteolytic degradation. Proteases of microbial origin
may be excluded by the use of freshly distilled water
and sterile filtration