UC知道拜托帮我下面这段话,是关于生物的(不要再线翻译的),感激不尽,急!!!
来源：百度知道 编辑：UC知道 时间：2024/06/17 17:26:42
Levels of YCA1 transcript are higher in Kllsm4D1
We previously suggested that, in the Kllsm4D1 mutant, the stabilization of mRNAs could lead to the abnormal production of some proteins that trigger apoptosis (Mazzoni et al, 2003b). In fact, the rate at which a specific mRNA is degraded can be a significant contributing factor to the overall expression levels of the encoded protein, and it has been recently reported that mRNA decay mutants show defects in translational control (Holmes et al, 2004).
As the absence of YCA1 could suppress most of the apoptotic phenotypes in Kllsm4D1 cells, we investigated whether YCA1 mRNA was also stabilized in this mutant. We prepared total RNA from exponentially growing and stationary cultures of wild-type and Kllsm4D1 cells and analysed the amount of YCA1 transcript. As shown in Fig 4A, we observed that the amount of YCA1 mRNA was more abundant in the mutant than in the wild type, both in exponential and stationary cells, suggesting the poss
We previously suggested that, in the Kllsm4D1 mutant, the stabilization of mRNAs could lead to the abnormal production of some proteins that trigger apoptosis (Mazzoni et al, 2003b). In fact, the rate at which a specific mRNA is degraded can be a significant contributing factor to the overall expression levels of the encoded protein, and it has been recently reported that mRNA decay mutants show defects in translational control (Holmes et al, 2004).
As the absence of YCA1 could suppress most of the apoptotic phenotypes in Kllsm4D1 cells, we investigated whether YCA1 mRNA was also stabilized in this mutant. We prepared total RNA from exponentially growing and stationary cultures of wild-type and Kllsm4D1 cells and analysed the amount of YCA1 transcript. As shown in Fig 4A, we observed that the amount of YCA1 mRNA was more abundant in the mutant than in the wild type, both in exponential and stationary cells, suggesting the poss
高水平的成绩单Kllsm4D1 YCA1中
我们先前的建议,在Kllsm4D1突变,稳定mRNAs可能会导致异常生成的蛋白质,触发凋亡(Mazzoni李玮,2003b)。事实上,这个比率下降mRNA具体可显著因素的综合表达水平的编码的蛋白质,它最近报道显示缺陷mRNA腐烂突变平动控制(福尔摩斯李玮,2004年)。
缺少YCA1可以抑制凋亡大部分细胞表型中,我们考察Kllsm4D1 mRNA YCA1是否也稳定在这个突变。我们准备指数增长从总RNA和固定的文化分析和细胞和Kllsm4D1野生YCA1量的成绩单。如图4A,,我们观察到的更丰富YCA1 mRNA突变比野生型的,无论是在指数和固定细胞的可能性,提出了稳定的成绩单的变异可能会导致循序渐进地积累和触发凋亡YCA1。
各级YCA1全文较高Kllsm4D1
我们曾建议，在Kllsm4D1突变，稳定的基因可能导致一些不正常的生产蛋白质触发细胞凋亡（崇义等人， 2003年） 。事实上，利率维持在一个特定的mRNA的降解可能是一个重要因素的总体表达水平的编码的蛋白质，并已最近报告说，基因突变体衰变缺陷平移显示控制（霍姆斯等人， 2004年） 。
由于缺乏YCA1能抑制多数凋亡细胞表型的Kllsm4D1 ，我们调查是否YCA1表达也稳定在这一突变。我们准备总RNA成倍增长和固定的文化野生型和Kllsm4D1细胞并分析了大量YCA1笔录。所显示的图4a ，我们观察到的数额YCA1表达更丰富的突变体比野生型，无论是在指数和固定细胞，暗示的可能性，即稳定的笔录中的突变可能导致逐步积累Yca1并触发细胞凋亡。