谁来帮我翻译一下这段500字的医学摘要呀？

来源：百度知道 编辑：UC知道 时间：2024/05/06 16:03:01
作为肺结核的一项临床检测，痰直接涂片检测法一直存在着阳性率较低的缺点，另外常用的4%NaOH消化液很容易由于放置时间未控制好而将细菌杀死。本课题通过改良痰液处理和检测中的一些步骤和方法，试图在不延长检测时间的情况下提高临床涂片的阳性检出率，并减少由于处理不当而造成细菌死亡的阴性率。通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。因此，我们认为以2%NaOH-NALC作为消化液、用磁性纳米颗粒进行富集后通过金胺O荧光染色观察的一套磁性纳米颗粒富集TB并检测的方法会比传统涂片和培养法有更高的敏感度。

关键字：结核分枝杆菌，TB，磁性纳米颗粒，肺结核

As a clinical detection of pulmonary tuberculosis, sputum smear test directly there has been a relatively low positive rate of the shortcomings of other commonly used digestive juice of 4% NaOH is easy to control as a result of storage time did not kill the good bacteria. This issue through an improved detection in sputum processing and some of the steps and methods, do not attempt to extend the detection time to improve the clinical detection rate of smear positive, and reduce the bacteria caused by improper handling of the negative rate of death. Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and au