UC知道英文翻译 英文译成中文
来源：百度知道 编辑：UC知道 时间：2024/05/28 07:54:40
Measurement of OVA-specific antibody
OVA-specific IgG2a and IgG2b antibodies in serum were detected
by ELISA according to the method previously described by
Sjoelander et al. with some modifications (Sun & Liu, 2008). In
brief, the wells of 96-well microtiter plates were coated with
100 ll of OVA solution (50 lg/ml in 50 mM carbonate buffer, pH
9.6) for 24 h at 4 C. The wells were washed 3 times with 200 ll
of PBS containing 0.05% (v/v) Tween 20 (PBS/Tween), and then
blocked with 200 ll of 5% FCS/PBS at 37 C for 1 h. After three
washings with PBST, 100 ll of diluted serum samples (IgG2a,
1:100; IgG2b, 1:50) or 0.5% FCS/PBS as control was added to triplicate
wells. The plates were then incubated for 1 h at 37 C, followed
by washing 3 times. Aliquots of 100 ll of horseradish
peroxidase-conjugated goat anti-mouse IgG2a or IgG2b (diluted
1:4000 in FCS/PBS) were added and incubated for 1 h at 37 C. After
washing,
OVA-specific IgG2a and IgG2b antibodies in serum were detected
by ELISA according to the method previously described by
Sjoelander et al. with some modifications (Sun & Liu, 2008). In
brief, the wells of 96-well microtiter plates were coated with
100 ll of OVA solution (50 lg/ml in 50 mM carbonate buffer, pH
9.6) for 24 h at 4 C. The wells were washed 3 times with 200 ll
of PBS containing 0.05% (v/v) Tween 20 (PBS/Tween), and then
blocked with 200 ll of 5% FCS/PBS at 37 C for 1 h. After three
washings with PBST, 100 ll of diluted serum samples (IgG2a,
1:100; IgG2b, 1:50) or 0.5% FCS/PBS as control was added to triplicate
wells. The plates were then incubated for 1 h at 37 C, followed
by washing 3 times. Aliquots of 100 ll of horseradish
peroxidase-conjugated goat anti-mouse IgG2a or IgG2b (diluted
1:4000 in FCS/PBS) were added and incubated for 1 h at 37 C. After
washing,
测量卵子，特异性抗体
卵清蛋白特异性IgG2a和IgG2b抗体的血清进行检测
用ELISA法根据先前所描述的方法
Sjoelander等。与一些修改（星期日及刘， 2008 ） 。在
简言之，井96孔微量板涂层
100名当地雇员卵子解决方案（ 50 LG电子/毫升在50毫米的碳酸盐缓冲液， pH值
9月6日）为24小时4角的水井洗3次， 200名当地雇员
的磷酸盐缓冲液含有0.05 ％ （ V / V ）的吐温20 （磷酸盐缓冲液/吐温） ，然后
封锁与200名当地雇员的5 ％的FCS /磷酸盐缓冲液在37 C的1小时经过三年
冲洗与PBST ， 100名当地雇员稀释血清样品（ IgG2a ，
1:100 ; IgG2b ， 1:50 ） ，或0.5 ％的FCS /磷酸盐缓冲液作为对照增加了三份
水井。板，然后孵育1小时37 ç ，其次
洗3次。
等分试样100镑辣根
过氧化物酶偶联羊抗小鼠IgG2a或IgG2b （稀释
1:4000功能界别/磷酸盐缓冲液）加和孵育1小时后在37角
洗衣机， 100镑的四甲基联苯胺（ 3,30,5,50 -四甲基联苯胺）液体
底增加了每口井。该板孵育
15分钟在37 C和酶反应而终止增加
50 11 / 2不适用的以及硫酸。吸光度测定使用
酶联免疫吸附器在490纳米与595纳米参考。数据表示
作为平均OD值减去样本平均
OD值的控制。结果表示了log2滴度。
凡套血清样本受到内部和
组间比较， ELISA法检测的所有样本
表现在同一天。