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Cell proliferation assay
Human hepatocellular carcinoma HEP-G2 and human intestinal
cancer HCT-22 cells were obtained from the Pharmacology Experimental
Center of Jilin University. HEP-G2 and HCT-22cells were
cultured in RPMI-1640 medium supplemented with 10% heat-inactivated
fetal bovine serum (FBS), penicillin (100 IU/ml) and streptomycin
(100 lg/mL) (Cui et al., 2007; Hsu, Kuo, & Lin, 2004;
Tomatsu, Ohnishi-Kameyama, & Shibamoto, 2003) under an atmosphere
of 5% CO2 at 37 C. The proliferation of HEP-G2 and HCT-22
cells was determined using colorimetric 3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl tetrazolium bromide (MTT) assay as described
previously (Cui et al., 2007). Briefly, an aliquot of 100 lL of cells
(105/mL) was attached for 24 h in the 96-well plate, and then removed
the supernatant. The polysaccharide (50, 100, 200,
400 lg/mL, final concentration) was seeded into each well for
48 h. The negative con
Human hepatocellular carcinoma HEP-G2 and human intestinal
cancer HCT-22 cells were obtained from the Pharmacology Experimental
Center of Jilin University. HEP-G2 and HCT-22cells were
cultured in RPMI-1640 medium supplemented with 10% heat-inactivated
fetal bovine serum (FBS), penicillin (100 IU/ml) and streptomycin
(100 lg/mL) (Cui et al., 2007; Hsu, Kuo, & Lin, 2004;
Tomatsu, Ohnishi-Kameyama, & Shibamoto, 2003) under an atmosphere
of 5% CO2 at 37 C. The proliferation of HEP-G2 and HCT-22
cells was determined using colorimetric 3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl tetrazolium bromide (MTT) assay as described
previously (Cui et al., 2007). Briefly, an aliquot of 100 lL of cells
(105/mL) was attached for 24 h in the 96-well plate, and then removed
the supernatant. The polysaccharide (50, 100, 200,
400 lg/mL, final concentration) was seeded into each well for
48 h. The negative con
细胞增殖试验
人肝癌细胞株Hep - G2和人体肠道
癌症细胞移植- 22细胞，从药理学实验
中心的吉林大学。喉癌Hep - G2和红细胞压积， 22cells人
培养1640 - 1640培养基中附加10 ％热灭活
胎牛血清（胎牛血清） ，青霉素（ 100国际单位/毫升） ，链霉素
（ 100 LG电子/毫升） （崔天凯等。 ， 2007 ;许，郭，与林， 2004年;
Tomatsu ， Ohnishi ，龟山，与柴， 2003年）的气氛下
5 ％ ，二氧化碳在37角的扩散株Hep - G2和红细胞压积- 22
细胞用比色法测定3 - （ 4,5 - dimethylthiazol - 2 -
基） -2,5 -二苯基四甲基溴化铵（ MTT法）检测所描述
以前（崔天凯等。 ， 2007年） 。简单地说， aliquot 100介细胞
（ 105/mL ）附24小时在96板，然后删除
上清液。多糖（ 50 ， 100 ， 200 ，
400 LG电子/毫升，终浓度）的种子在每个好
48小时在阴性对照治疗中只。
约10.0介0.4 ％ MTT法加入每个以及培养
另外4小时后上清液吸气， 100介Me2SO
添加到文化和趋同至少10分钟
充分溶解有色材料。吸光度在570纳米
测定的酶联免疫吸附试验器（型号680 ，酶标仪文书） 。抑制率处理的细胞的基础上计算公式如下： （ 1 - A570值处理的细胞/的A570价值未处理细胞） * 100 ％ 。