谁能帮忙翻译一下这段论文

来源:百度知道 编辑:UC知道 时间:2024/05/23 23:00:30
植物组织培养,是根据植物细胞具有全能性的理论,利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织(如形成层、表皮、皮层、髓部细胞、胚乳等)或细胞(如大孢子、小孢子、体细胞等)以及原生质体,在无菌和适宜的人工培养基及光照、温度等人工条件下,能诱导出愈伤组织、不定芽、不定根,最后形成完整的植株的学科。
酿酒葡萄病毒种类繁多,脱毒后容易重新感染,因此在酿酒葡萄组织培养过程中病毒的脱除,脱毒后对脱毒效果的检测以及如何避免脱毒植株重新感染病毒就成为了关键和难点之一。酿酒葡萄病毒脱除方法主要有茎尖培养、热处理脱毒、抗病毒药剂法等,鉴于实验室条件有限本实验主要采用茎尖培养对酿酒葡萄进行病毒的脱除,脱毒效果比较理想。
本实验以酿酒葡萄的顶芽为试验材料,研究了消毒条件、植物生长调剂、顶芽大小等因素对丛生芽再生、扩繁、生根、脱毒等的影响。结果表明:取芽大小激素的浓度对丛生芽再生、扩繁、生根都起着重要的作用,对植株脱毒也起着重要的作用,茎尖在25 ℃、16h/8h光/暗周期条件下,以MS+6-BA1.0mg/l+NAA0.1mg/l诱导培养基培养获得不定芽,经继代、生根培养,获得了正常的无毒植株。

Plant tissue culture, is based on the theory of plant cells, showing the plant organs (such as from roots, stems and leaves, flowers, fruits meristem-tip, etc), the organization (e.g., skin, cortex, cambium marrow cells, endosperm, etc.) or cell (such as megaspore and microspore, etc.) and protoplast, sterile and appropriate artificial medium and light, temperature and condition, can induce callus and in vitro shoot, adventitious roots, and finally forming a complete plant.
Grape variety, virus infection, easy to after virus-free in grape tissue culture, the virus after removal of virus-free effect of virus-free detection and how to avoid virus-free plant virus infection to become one of the key and difficult. Grape virus removal methods are mainly meristem-tip culture, heat treatment reagent method, antiviral virus-free in the laboratory condition, this experiment mainly adopts limited meristem-tip culture of wine grape, virus of virus-free ideal effect.
This experiment w