UC知道有关lacZ
来源：百度知道 编辑：UC知道 时间：2024/06/06 00:26:35
LacZ’: encoding the N-terminal -peptide of galactosidase, which can complement the C-terminal -fragment of galactosidase produced in a host strain (E.coli.DH5) to hydrolyze lactose glucose + galactose, or 5-bromo-4-chloro-3-indolyl--D-galactopyrunoside (X-gal) blue color product.
LacZ’ is repressed by lacI repressor and can be induced by isopropyl--D-thiogalactopyrunoside(IPTG).谁能帮忙翻译一下
LacZ’ is repressed by lacI repressor and can be induced by isopropyl--D-thiogalactopyrunoside(IPTG).谁能帮忙翻译一下
LacZ：编码半乳糖甘酶的N端，它可以和宿主菌株（DH5α）产生的半乳糖甘酶C端互补，将乳糖分解为葡萄糖和半乳糖，或将5-溴-4-氯-3-吲哚-β-D-半乳糖（X-gal）分解为蓝色产物。
LacZ被lacI抑制子所抑制，可以被异丙基-β-D-硫代半乳糖苷 （IPTG）所诱导。