树上没有鸟

There is no birds in the tree
或
No birds are in the tree
或
The tree is with no birds

on:树本身的产物，如果子呀
in:与上面的on相反

there's no bird in the tree
树本身长出来的用on，外物用in

There are no birds in the tree

There is no bird in the tree
我忘了是用in还是on 自己查查吧

There are no birds in the tree.

No bird on the tree