用DNAman分析引物时，Thermo Tm、Hybridization Tm、GC+AT Tm分别是什么意思？急！！！

The melting temperature of an oligonucleotide is
calculated with three methods:

1)Thermodynamic Tm:

The Tm is calculated using the nearest-neighbor
thermodynamic values method (Breslauer et al). This method is less accurate for
longer oligos. The formula for the Tm is

Tm=dH/(dS-Rln(Cd))-273.15+16.6*(log10(Cs))

Where dH is the enthalpy, dS is the
entropy, R is 1.987 cal K-1 mol-1, Cd is the
DNA concentration, and Cs is the salt concentration.

2)Hybridisation Tm:

This method is generally used for DNA or RNA
hybridization, especially in presence of high salt and formamide. It is more
accurate with longer oligos. The Tm is calculated using the following
formula:

for DNA:DNA hybridization:

Tm=81.5+16.6*(log10[Na+])+0.41*[%(G+C)]-0.63*(%Formamide)-500/L-1.5(%Mismatch)

for DNA:RNA hybridization:

Tm=79.8+18.5*(log10[Na+])+0.58*[%(G+C)]