UC知道急求帮忙翻译!!!(4)
来源：百度知道 编辑：UC知道 时间：2024/05/06 00:52:20
Construction of swine macrophage cDNA libraries. cDNA libraries were generated
from mRNA isolated from ASFV-infected and uninfected macrophage
cell cultures (60 75-cm2 flasks) prepared from swine peripheral blood mononuclear
cells (19). Macrophages were infected with pathogenic ASFV isolate Pr4 at
a high multiplicity of infection (MOI) of 20, and at 3, 6, and 18 h postinfection
(hpi), infected cells were harvested and lysed. RNA was extracted with acid
phenol-chloroform and precipitated with isopropanol. Total RNA was purified
by LiCl precipitation, and poly(A) RNA transcripts were enriched by two successive
rounds of oligo(dT) affinity chromatography (28). Five micrograms of
mRNA was used to generate directional cDNA libraries with commercially
available cDNA synthesis and cloning kits (Superscript II system; Life Technologies).
Approximately 15,000 cDNA clones were sequenced and characterized by
comparison to genetic databas
from mRNA isolated from ASFV-infected and uninfected macrophage
cell cultures (60 75-cm2 flasks) prepared from swine peripheral blood mononuclear
cells (19). Macrophages were infected with pathogenic ASFV isolate Pr4 at
a high multiplicity of infection (MOI) of 20, and at 3, 6, and 18 h postinfection
(hpi), infected cells were harvested and lysed. RNA was extracted with acid
phenol-chloroform and precipitated with isopropanol. Total RNA was purified
by LiCl precipitation, and poly(A) RNA transcripts were enriched by two successive
rounds of oligo(dT) affinity chromatography (28). Five micrograms of
mRNA was used to generate directional cDNA libraries with commercially
available cDNA synthesis and cloning kits (Superscript II system; Life Technologies).
Approximately 15,000 cDNA clones were sequenced and characterized by
comparison to genetic databas
猪巨噬细胞cDNA图书馆的建筑。 cDNA图书馆从与ASFV被传染的和未感染的巨噬细胞细胞培养隔绝的mRNA引起了(60 75cm2烧瓶)准备从猪周边血液单核细胞(19)。巨噬细胞感染致病性ASFV孤立Pr4在一种高多样性传染(MOI) 20，并且在3， 6和18 h postinfection(hpi)，被传染的细胞被收获了并且被溶解了。 RNA提取了与酸酚三氯甲烷并且沉淀了与异丙醇。总RNA由LiCl降雨雪净化，并且多(A) RNA抄本由二个连续圆oligo (dT)亲合色谱法丰富(28)。 五微克mRNA用于引起定向cDNA图书馆与商业可利用的cDNA综合和克隆成套工具(上标II系统; 生活技术)。 大约15,000 cDNA克隆程序化并且描绘为与基因数据库(J.的比较。 Neilan，未出版的数据)。七千七百十二cDNA克隆从二个cDNA图书馆(2,925从一个ASFV被传染的巨噬细胞图书馆和4,787从一个没被感染的巨噬细胞图书馆)由Blast分析选择以切除值为200 (2)并且用于修建cDNA microarray。
-----------------
软件翻译的，只作参考