UC知道生物英语,谁能帮忙翻译一下
来源：百度知道 编辑：UC知道 时间：2024/06/01 13:08:26
In the examples described above the unnatural amino acid had to be exogenously
added to the medium. Thus, a transport system for the unnatural amino
acid was required. To bypass this requirement and create an organism with a true
21 amino acid genetic code, genes for the biosynthetic pathway for p-aminophenylalanine
(pAF), as well as a unique pAF-AARS and cognate tRNApAF, were
introduced into E. coli (103). The pAF biosynthesis genes came from the
chloramphenicol-producing Streptomyces venezualeae. By combining the biosynthetic
pathway for the analog with the analog-specific AARS, Schultz and
coworkers created an organism that, under induction with IPTG, can autonomously
introduce pAF at all amber codons. This approach cannot at present be
used for sustained growth of the organism.
谢谢了!
added to the medium. Thus, a transport system for the unnatural amino
acid was required. To bypass this requirement and create an organism with a true
21 amino acid genetic code, genes for the biosynthetic pathway for p-aminophenylalanine
(pAF), as well as a unique pAF-AARS and cognate tRNApAF, were
introduced into E. coli (103). The pAF biosynthesis genes came from the
chloramphenicol-producing Streptomyces venezualeae. By combining the biosynthetic
pathway for the analog with the analog-specific AARS, Schultz and
coworkers created an organism that, under induction with IPTG, can autonomously
introduce pAF at all amber codons. This approach cannot at present be
used for sustained growth of the organism.
谢谢了!
在上面的例子中所述的非自然的氨基酸都必须人为的添加到中等. 因此,一个传动系统是非自然的氨基酸的必须. 绕过这一要求,并营造一个有机体与一个真实的21个氨基酸的遗传密码, 基因生物合成途径的苯基丙氨酸,以及独特的paf - aars同源trnapaf , 导入大肠杆菌( 2335 ) . 扶贫合成的基因来自氯霉素产生链霉菌venezualeae . 结合生物合成途径的模拟与模拟具体aars ,舒尔茨和同事创造了一个有机体, 在IPTG诱导后,可自主引进paf在所有黄色的密码. 这种方法目前不能用于持续增长的有机体.