哪位大虾能帮小弟翻译下这篇摘要，急，毕业论文要用的，谢谢！

来源：百度知道 编辑：UC知道 时间：2024/05/22 13:47:18
海洋酵母菌SOD分离纯化的初步研究

摘 要：以海洋酵母菌为材料，采用甲苯法、乙醇－氯仿法、异丙醇法和超声波法对其进行破壁；分离出SOD粗酶液，采用邻苯三酚自氧化法，测定不同破壁方法下SOD粗酶液酶活，选取酶活最高的甲苯法；将菌体用甲苯法破壁得到的粗酶液调节pH除杂蛋白，丙酮二次沉淀后，得到纯化的SOD，其比活为1219 U/mg，收率为5.6%；在25℃~95℃间对SOD进行耐热性实验，结果表明低于55℃时SOD活性基本稳定，65℃时仍能保持79.64%的酶活；纯化后SOD经CHCl3-乙醇，H2O2抑制试验，酶类型为Cu、Zn-SOD；在200~400nm 紫外光下测定酶的最大吸收波长，260 nm时出现最大OD值0.931，呈现出波峰，证明酶类型为Cu、Zn-SOD。

关键词：超氧化物歧化酶 海洋酵母 破壁方法 分离纯化 SOD类型
Keywords: Superoxide dismutase; marine yeast;
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SOD marine yeast Purification of a preliminary research

Abstract : marine yeast for materials and the use of toluene, ethanol-chloroform, isopropanol and ultrasonic its broken; SOD isolated from crude enzyme solution, using pyrogallol autoxidation, Determination of different methods under broken SOD enzyme activity, select the highest activity of toluene; Toluene used to cell was broken by the enzyme regulating pH protein removal, acetone secondary precipitation, SOD be purified, to live more than 1,219 U / mg, yield of 5.6%; at 25 ° C to 95 ° C between the SOD heat for experiments, The results showed less than 55 ° C, SOD activity was basically stable, at 65 ° C can maintain 79.64% of the activity; SOD purified by CHCl3-ethanol, hydrogen peroxide inhibition test, the enzyme types of Cu, Zn - SOD; 200 ~ 400 nm in the UV-determination under the maximum absorption wavelength, 260 nm see the largest OD value of 0.931, showing peaks and proof-type Cu, Zn - SOD.

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