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来源：百度知道 编辑：UC知道 时间：2024/05/07 08:12:50
1、免疫亲和微柱-荧光测定法是在免疫亲和层析柱—荧光光度计法（IAC-SFB）的基础上，用自主研发的免疫亲和微柱取代进口免疫亲和柱的一种黄曲霉毒素快速定量检测法，并经免疫亲和柱-荧光光度计法（IAC- SFB）即美国VICAM公司真菌毒素荧光分析仪验证此定量法，AFT回收率均在80%以上，变异系数处于10%以内，说明该方法测定结果准确可靠，重复性良好。
2、本研究又在黄曲霉毒素快速定量检测方法的基础上，结合简易荧光测定仪和吸附柱，发展了黄曲霉毒素快速定性检测法，即免疫亲和微柱-吸附柱-荧光测定法（IAC-SM-SFB）。各项性能指标表明，在空白样品中进行AFT添加回收实验的检测限1ppb～2ppb，低于国家标准中所规定的5ppb ；进行AFM1添加回收实验的检测限为0.2ppb～0.3ppb，低于国家标准中所规定的0.5ppb，均能够满足国家标准的检测要求。并且用免疫亲和微柱-荧光测定法验证此定性法，具有灵敏度高、快速、安全、操作简易、价格低廉、特异性好、适用性广、高通量等特点。
3、称取各种样品用本实验建立的两种黄曲霉毒素快速检测方法检测其中的黄曲霉毒素污染情况，两种方法的检测结果基本相符。说明此两种方法可进行AFT的测定，定量法可适合对AFT污染进行确证，定性法能够符合现场筛查要求。

An immune-and micro-column - fluorescence method is immune affinity chromatography column - Fluorescence Spectrometer (IAC-SFB) base, using self-developed immune pro - and micro-Immunoaffinity replace imports and, in a aflatoxin rapid quantitative detection, and the immune affinity column - Fluorescent Light meter (IAC-SFB) that the United States VICAM company fungal toxin fluorescence analyzer test this quantitative method, AFT recoveries were 80% and above, the coefficient of variation is less than 10%, indicating that the measured results are accurate and reliable, good repeatability.
Two, this study also aflatoxin rapid quantitative detection method based on the simple combination of fluorescence analyzer and adsorption column, the development of rapid detection of aflatoxin, which pro - and micro-immune - adsorption column - fluorescence assay (IAC-SM-SFB). The performance indicators show that the blank samples for the recovery of AFT add roughly ~ ppb detection limit, be