求助分子生物学论文摘要的英文翻译

来源：百度知道 编辑：UC知道 时间：2024/05/16 08:01:10
【摘要】目的：了解作业工人外周血淋巴细胞p53及DNA损伤修复相关基因mRNA表达水平与苯暴露环境关系，探讨特异性、敏感性、稳定性好，标本易采集等优点检测指标，应用于苯职业人群健康监护。方法：应用SYBR GreenI嵌合荧光法实时荧光定量RT-PCR分析技术，检测72名作业工人（根据岗位不同分为A组、B组）和29名对照人员外周血淋巴细胞p53及相关基因mRNA表达水平。应用差异表达分析软件REST 2005，比较对照组与作业组间表达倍数差异；同时进行外周血白细胞、血红蛋白和血小板计数；抽检原料甲苯、喷涂剂中苯含量；车间空气三苯浓度监测。结果：A组和B组p53、p21、ku80、Ape1和Mdm2表达水平与对照组比较差异无显著性，A组的Rad51、Bcl-2、Bax、Xpa和Xpc表达水平下调、B组Rad51表达下调与对照组比较有统计学意义。A组的白细胞、血红蛋白和血小板低于对照组，B组血小板低于对照组差异有显著性。结论：苯作业工人血细胞中部分与DNA损伤修复相关基因的mRNA表达水平与非接苯对照组比较存在差异。SYBR GreenI嵌合荧光实时RT-PCR法与REST 2005统计软件，检测和分析基因mRNA差异表达水平方法，适用于职业人群的分子流行病学研究及健康监护。

Abstract Objective : Workers peripheral blood lymphocytes of p53 and DNA damage and repair gene mRNA expression levels of benzene exposure and the environment, explore specificity, sensitivity, stability, easy to collect specimens of the advantages of detecting indicators used benzene health care professional groups. Methods : SYBR GreenI chimeric fluorescence quantitative real-time fluorescence RT-PCR analysis, testing workers exposed to 72 (under different posts divided into Group A, Group B) and 29 staff were peripheral blood lymphocytes and related gene p53 mRNA expression level. Application of differential expression analysis software REST 2005, compared with the control group operating multiple groups to express differences; While peripheral blood leukocytes, hemoglobin and platelet count; Sampling of raw materials toluene, benzene spraying agent content; Workplace air toluene concentration monitoring. Results : Group A and Group B p53, p21, wild, Ape1 and Mdm2 expression leve