基因转化---短文翻译！

来源：百度知道 编辑：UC知道 时间：2024/06/20 19:02:51
以原位生殖器官(包含花粉管，受精前后的配子和合子)为转基因受体。在对油菜授粉后的花柱和子房进行细胞学观察的基础上，确定遗传转化的操作时间，在该时间内，削去柱头，滴加外源DNA溶液，使携带外源目标基因和草胺膦抗性基因（bar）为标记基因的DNA对生殖细胞进行转化，通过生长发育，获得转基因的T1代合子（种子），在T1代幼苗早期用低浓度的除草剂草胺膦进行喷洒筛选，获得转基因阳性油菜植株。对于不同的油菜品种转基因效率在2～10%。

In situ genital mutilation (including pollen tube, fertilization before and after the gametes and zygotes) transgenic receptor. Rape after pollination in the style and ovary Cytological observation conducted on the basis of genetic transformation determine hours of operation, in the time, the defrocked Stigma, dropping exogenous DNA solution, the goal of carrying exogenous gene and grass amine phosphine resistance gene (bar) for the DNA marker genes of reproductive cells transformed by growth and development, access to the T1 generation transgenic homozygous (seeds), T1 and early seedling with low concentrations of herbicides were sprayed grass amine phosphine screening, access positive transgenic rapeseed plants. The different varieties of transgenic rapeseed efficiency in the 2 ~ 10%