化学文献翻译20分一段~第一段

来源：百度知道 编辑：UC知道 时间：2024/06/12 13:52:41
2.3 Physical measurements
1H NMR spectra were recorded on an Avance-400 spectrometer with d6-DMSO (dimethyl sulfoxide) as the solvent at room temperature and TMS (tetramethylsilane) as the internal standard. UV–Vis (UV–visible) spectra were recorded on a Perkin–Elmer Lambda-25 spectrophotometer, and emission spectra were recorded on a PerkinElmer LS-55 luminescence spectrometer at room temperature. Electrospray mass spectra (ES-MS) were recorded on a LQC system (Finngan MAT, USA) using CH3CN as the mobile phase.
The absorption titration of ruthenium(II) complexes in buffer was carried out by adding the DNA stock solution to a fixed concentration of ruthenium. Ruthenium solutions employed were 10 μM in concentration and calf thymus DNA was added to a ratio of 1.27:1 [DNA]/[Ru]. Ruthenium-DNA solutions were allowed to incubate for 5 min before the absorption spectra were recorded. The intrinsic binding constants Kb of Ru (II) complexes to DNA were calculated from equation [14]:

2.3物理测量
核磁共振谱记录的进步 - 400谱仪与D6中-二甲基亚砜（二甲亚砜）为溶剂，在室温和全方位维修计划作为内部标准。紫外可见光谱（紫外可见）谱记录的一珀金-埃尔默的LAMBDA - 25分光光度计，和发射光谱均录得就一珀金埃尔默的LS - 55发光光谱仪在室温下。电喷雾质谱（安老服务- MS ）的均录得就一lqc系统（ finngan垫，美国）使用乙腈作为流动相。
吸收滴定法测定钌（二）配合在缓冲区进行了加入的DNA原液，以一个固定的浓度钌。钌解决方案，聘请10 μ m的浓度和小牛胸腺DNA ，加入的比例为1.27:1 [ DNA的] / [如] 。钌- DNA的解决方案，获准以孵化为5分钟前，吸收光谱均录得。内在的结合常数KB的茹（二）配合物的DNA计算从方程[ 14 ] ：
[ DNA的] / （ ε 1 -六） = [ D NA的] / （  B组- 六） + 1 / [ K B （下 B组-  f）项]
其中[ DNA的]是浓度的DNA的碱基对， ε 1 ， ε f和ε b对应的表观吸收系数aobsd / [如] ，消光系数为钌配合物在完全约束的形式，分别为。在图谋[ DNA的] / （ ε 1 -ε f ）在银两[ D NA的] ，或给出的比例斜坡向拦截。
粘度测量所进行的使用乌伯娄德粘度计粘度保持温度恒定在28.0 （ ± 0.1 ）  C在恒温水浴。电脑断层的DNA样本约200个碱基对在平均长度分别编写的sonicating ，以尽量减少复杂性所引起的DNA的灵活性， [ 15 ] 。流动时间是衡量一台数码秒表，每个样品测量的3倍，平均流通时间计算。数据作为（  /  0 ） 1 / 3银两约束力的比率[ 16 ] ，其中是粘度的CT - DNA的存在复杂，  0是粘度的CT - DNA的单。

.3物理测量
1H核磁共振光在与d6-DMSO (二甲亚砜)的一台Avance-400分光仪被记录了作为在室温和TMS (tetramethylsilane)的溶剂作为内部标准。 UV–Vis (UV–vi