UC知道哥哥姐姐帮忙翻译个，谢谢了啊
来源：百度知道 编辑：UC知道 时间：2024/06/07 19:05:42
Effects ofaeration on the activity and distribution
of the enzyme. Since the results cited above indicated
a relationship between aeration and the
activity and distribution of the enzyme, it was of
interest to determine whether anaerobically
grown cells would be affected by subsequent
aeration. For this purpose, resting cellular suspensions
obtained from standing cultures were
aerated in 0.1 M phosphate buffer for 24 hr after
they were harvested. These cells showed no changes in the distribution of the enzyme among
the major cellular fractions, whether they had
been aerated in the absence of an energy source
or in the presence of 2% glucose, 0.5% succinate,
or 0.5% acetate.
In contrast, when cells were grown under
aerobic conditions and were tested for acetyl-CoA
synthetase, a rather complex behavior was observed.
First of all, there was a steady increase,
with time of incubation
of the enzyme. Since the results cited above indicated
a relationship between aeration and the
activity and distribution of the enzyme, it was of
interest to determine whether anaerobically
grown cells would be affected by subsequent
aeration. For this purpose, resting cellular suspensions
obtained from standing cultures were
aerated in 0.1 M phosphate buffer for 24 hr after
they were harvested. These cells showed no changes in the distribution of the enzyme among
the major cellular fractions, whether they had
been aerated in the absence of an energy source
or in the presence of 2% glucose, 0.5% succinate,
or 0.5% acetate.
In contrast, when cells were grown under
aerobic conditions and were tested for acetyl-CoA
synthetase, a rather complex behavior was observed.
First of all, there was a steady increase,
with time of incubation
影响ofaeration对活动和分配
的酶。由于上述结果表明，
一之间的关系曝气和
活动和分布的酶，它是
利息，以确定是否厌氧
细胞的生长会受到影响，它的利益，以确定是否厌氧细胞的生长会受到影响，随后曝气。为此，休息细胞悬浮液获得由常委会文化被曝气在0.1米磷酸盐缓冲液24小时后
他们收获了。这些细胞显示没有发生变化的分布酶之间
主要的细胞形态，他们是否有
一直曝气，在缺乏能源的来源
或在该律师在场的2 ％ ，葡萄糖， 0.5 ％ ，琥珀，
或0.5 ％ ，醋酸。
在此相反，当细胞下生长
在好氧条件和测试为乙酰辅酶A
合成酶，相当复杂行为的观察。
首先，有一个稳步增加，
随着时间的孵化，在具体的活动
酶。此外，发生广泛的变化
在细胞分布的酶
孵化过程中。在指数期
的增长，大部分的酶沉积与
该微粒分数，很少被发现
在线粒体的一部分。之后，细胞
进入平稳阶段，但是，大的增加
在活动的线粒体分数
观察（见表2 ） 。经过24小时的
孵化，大约80 ％的总
酶活性，在微粒分数，
只有微不足道的金额，在线粒体
材料。然而，当细胞进入
固定相，有一个急剧上升，
金额酶沉积与
线粒体，以便在48小时约70 ％的
活动是在现在这个分数，和％
总的活动，在微粒物质减少
相应地。经长期培育，
具体的活动线粒体
分数，以及其总酶活性，
下降到比较低的水平再次。在其他
实验这种类型的板计数作出
间隔在整个96小时的增长期
显示没有减少在活细胞数
在整个孵化期，因此，执政党
一般细胞退化作为一个解释
的影响后指出，长期培育。
细胞生长aerobically 0.5 ％钠
醋酸作为碳源，而不是2 ％葡萄糖增加细胞。该微粒分数获得
从醋酸成长的细胞在收获指数
和早期固定相（即，直至
大约24小时）载有大约70 ％的