UC知道求论文翻译,急!!!!!!
来源：百度知道 编辑：UC知道 时间：2024/09/22 07:58:43
2.1. Peptides
K19Hc and P18, a cecropin-magainin hybrid [17], were synthesized
using an automated solid-phase peptide synthesizer (Pioneer Applied
Biosystems, Foster, CA), at the Korea Basic Science Institute. The
Buforin II [18] used in this study was purchased from Sigma (St. Louis,
USA). All of the peptides were then re-purified via C18 reverse-phase
(RP) high-pressure liquid chromatography (HPLC). In order to prepare the di-K19Hc, the purified K19Hc was exposed to 0.1 M
ammonium bicarbonate (1 mg/ml) for at least 72 h at room temperature.
Fig. 1 shows the HPLC profiles of the purification of K19Hc
and di-K19Hc. The purity of the peptides was monitored via HPLC
profile and tricine SDS–PAGE analysis (right panel in Fig. 1) [19], conducted
under non-reducing conditions. The purified peptides were
completely dried and stored at 70 C until used. When necessary,
the peptides were dissolved in acidified distilled water (0.
K19Hc and P18, a cecropin-magainin hybrid [17], were synthesized
using an automated solid-phase peptide synthesizer (Pioneer Applied
Biosystems, Foster, CA), at the Korea Basic Science Institute. The
Buforin II [18] used in this study was purchased from Sigma (St. Louis,
USA). All of the peptides were then re-purified via C18 reverse-phase
(RP) high-pressure liquid chromatography (HPLC). In order to prepare the di-K19Hc, the purified K19Hc was exposed to 0.1 M
ammonium bicarbonate (1 mg/ml) for at least 72 h at room temperature.
Fig. 1 shows the HPLC profiles of the purification of K19Hc
and di-K19Hc. The purity of the peptides was monitored via HPLC
profile and tricine SDS–PAGE analysis (right panel in Fig. 1) [19], conducted
under non-reducing conditions. The purified peptides were
completely dried and stored at 70 C until used. When necessary,
the peptides were dissolved in acidified distilled water (0.
我先来抛砖引玉吧：
K19Hc和P18，一种抗菌肽和蛙皮肽的杂合物，利用韩国基础科学研究院的自动固相多肽合成仪（先锋公司）合成，实验中使用的BuforinII购自Sigma公司。所有实验中使用的多肽，都经过反向高效液相色谱C18柱再次纯化。纯化的K19Hc在室温下于0.1M的碳酸氢铵（以1mg/ml)处理72小时，以制备di-K19Hc.
图1.显示纯化的K19Hc和di-K19Hc 的高效液相色谱特征图。利用高效液相色谱和非还原条件的Tricine－SDS－PAGE来鉴定制备的多肽的纯度（图1右栏）。纯化的多肽经冻干后于－70度保存。用含0.01%冰醋酸的蒸馏水溶解制备的多肽，以牛血清白蛋白作为标准对照，利用BCA法（来自Pierce公司）来测定多肽浓度。
时间有限，我只能先翻一段。大家一起来帮忙。