翻译，急急，拜托各位了

来源：百度知道 编辑：UC知道 时间：2024/06/09 02:55:29
摘要

以新鲜山药为原料，研究山药中多酚氧化酶(PPO)的最适pH值、最适温度、最适底物浓度及添加剂的影响，设计了不同护色方案，以确立山药的最佳护色条件。采用分光光度法研究pH值、温度对酶活性的影响；比较不同护色液对酶活力的影响；结果表明，以邻苯二酚为底物，该酶最适作用pH 为5.8，最适作用温度为20℃，Km=1.82mol/L，Vmax=1.11(Δ OD/min)。本文同时考察了抑制剂对山药多酚氧化酶活性的影响，从而制定出最佳护色措施。

关键词：山药；多酚氧化酶；动力学特性；褐变；护色

Take the fresh Chinese yam as raw material, studies in the Chinese yam the polyphenoloxidase (PPO) most suitable pH value, the optimum temperature, the most suitable substrate concentration and the chemical additive influence, designed has protected the color plan differently, established the Chinese yam to protect the color condition best. Uses the spectrophotometric method to study the pH value, the temperature to the enzyme activity influence; Compared with protects the color fluid differently to the enzyme vigor influence; The result indicated that take the catechol as the substrate, this enzyme most is suitable affects pH is 5.8, most suitable function temperature for 20℃, Km=1.82mol/L, Vmax=1.11 (DELTA OD/min). This article simultaneously inspected the inhibitor to the Chinese yam polyphenoloxidase active influence, thus formulates protects the color measure best. key word: Chinese yam; Polyphenoloxidase; Dynamics characteristic; Turning brown; Protects the color

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