求教高人有关Agarose gel Electrophoresis 图分析的问题

来源：百度知道 编辑：UC知道 时间：2024/05/15 17:43:27
紧急：因为是考试的问题，所以一定要在16号以前回复，多谢！！！1. In figure 1， an agarose gel is depicted, in the First lane a DNA-ladder has been loaded containing DNA-fragments of 100, 200, 300, 400 and 500 bp. In the second lane the PCR product was loaded obtained using a patient sample and a primer pair giving rise to a product at 250 bp if genomic DNA of Mycobacterium tuberculosis is present. What can you conclude from this test? - Is the patient sick? Explain. - Is something missing in this test, explain what if so.- What are the possible problems and possible solutions when testing for infection using PCR?- Can you use this test to follow the efficacy of a therapeutic intervention, explain.Figure 1，因为发不了图片，所以另寻地址：http://hiphotos.baidu.com/%CE%D2%B0%AE%D3%F1%CA%F7%C1%D6%B7%E5/pic/item/c8ea5f4482e0b296b3b7dc45.jpg
最好能用英语，不用也没关系，因为我现在是一点头绪都没有呀！

The patient is sick because there is a 250 bp gel band presents, which indicates the DNA of Mycobacterium tuberculosis.
A control sample is missing in this test. Thus, we are not able to compare this potential patient's DNA sample with a healthy human DNA sample (a control), in order to confirm the pressence of disease in patient.
One of the major problem with PCR is contamination during experiment. This is because PCR is a sensitive technique and it requires extensive care in order to obtain accurate result.
I don't really sure about how to answer the last queation. However, I think tha answer should be CAN, because the effect of drug or treatment may cause the DNA fragment to change in its length, and consequently the gel band will shift to another location (no longer on the 250bp location).