nature文章翻译或大意

来源：百度知道 编辑：UC知道 时间：2024/06/15 01:55:38
文章来源：
《BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.》 作者：Ina Poser, Mihail Sarov et al .Nature Methods. 2008.Vol 5 No.5:409-415.
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《BAC TransgeneOmics ： 蛋白质作用的探险的高生产量方法在mammals.》 作者：Ina装腔作势者， Mihail Sarov等的。自然方法。 2008.Vol 5 No.5 ：409-415.

《BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.》 作者：Ina Poser, Mihail Sarov et al .Nature Methods. 2008.Vol 5 No.5:409-415.
《BAC 转基因：一种高量法探测哺乳动物中蛋白质的功能》
作者：Ina Poser, Mihail Sarov
2008.Vol 5 No.5:409-415.

The interpretation of genome sequences requires reliable and standardized methods to assess protein function at high throughput. Here we describe a fast and reliable pipeline to study protein function in mammalian cells based on protein tagging in bacterial artificial chromosomes (BACs). The large size of the BAC transgenes ensures the presence of most, if not all, regulatory elements and results in expression that closely matches that of the endogenous gene. We show that BAC transgenes can be rapidly and reliably generated using 96-well-format recombineering. After stable transfection of these transgen