UC知道高分急求！！！生物相关翻译-中译英
来源：百度知道 编辑：UC知道 时间：2024/06/19 23:56:54
本实验探讨研究了如何将原核表达的人载脂蛋白A-I，经过一系列的分离纯化步骤，通过常用的层析等方法，成功、高效、大量的分离出纯净的载脂蛋白A-I。试验结果表明，通过超声破菌后，离心取上清溶液，经离子交换层析以PB：PBS=**：**比例洗脱，进一步用镍柱亲和层析以***mM咪唑洗脱，用**酶25摄氏度水浴4小时酶切，再用亲和层析分离标签，最终所得穿透溶液经Western检测为较纯载脂蛋白A-I。本实验方法可代替从大量人血清中应用超速离心等传统的提取ApoA-Ⅰ的方法，具有良好的应用前景，为今后的工业生产提供了良好的基础。
This study was research for prokaryotic expression of human apolipoprotein A-1 how to successfully ,efficiently and plentifully isolate the pure apolipoprotein A-1 through a series of isoltation and purification by general chromatography .The result show the penetrated solution was for pure apolipoprotein A-1 by Western through bacteria break by ultrasound , centrifugated the suspension,and which by the ion-exchange chromatography to PB: PBS =**:** the ratio of elution, and using nickel affinity chromatography column to *** mM imidazole elution , ** enzyme digestion four hours in 250C water bath, and then isolated the labels by affinity chromatography . The method could replaced a large number of human serum which got ApoA-Ⅰ by traditional ultracentrifugation , has good application prospects to provided a good foundation for future industrial production.
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Studied in this experiment to explore how the original expression of human apolipoprotein AI, a series of s