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来源:百度知道 编辑:UC知道 时间:2024/06/22 19:37:02
L-色氨酸是人和动物生命活动中八种必须氨基酸之一,对人和动物的生长发育、新陈代谢起着重要作用。L-色氨酸的生产方法有多种,其中以发酵法最具发展前途。因此,如何采取有效措施提高发酵液中L-色氨酸的产率,并将其精制出来是目前急待解决的问题。本文通过对种树脂的筛选、吸附和洗脱条件的优化,以及对脱色、结晶方法的研究与摸索,最终确定强酸型阳离子树脂HZ-001在pH=3,3倍床体积流速,温度室温,对色氨酸有最大的吸附作用;将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱,再用2mol/L的氨水6倍床体积流速在室温下进行洗脱,收集洗脱液,60℃浓缩为过饱和状态后加入30%无水乙醇,4℃下100rpm摇起晶20h,过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。总体收率为72.36%,纯度达97.4%。

L-tryptophan is a kind of necessary amino acid.It is useful for the growth of people and animals.There are several production methods of L-tryptophan,ultimately only L-tryptophan production by fermentation proved commercially feasible. Therefore,increasing the production and extraction of L-tryptophan are the problems that needed to be settled at present. Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the lar

L-tryptophan is a human and animal life in one of eight amino acids necessary for human and animal growth and development, play an important role in metabolism. L-Tryptophan has a variety of production methods, of which the most promising fermentation. Therefore, how to take effective measures to improve the fermentation broth of L-tryptophan production rate, and is refined out of the question of the immediate problem. In this paper, the selection of the kinds of resin, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research and exploration, and ultimately determine the strong acid cation resin HZ-001 at pH = 3,3 times the bed volume flow rate, temperature, room temperature, maximum of tryptophan adsorption; will Centrifugal fermentation broth to precipitate after decolorization with activated carbon through the HZ-001 strong acid cation exchange resin column, and then 2mol / L of ammonia flow rate of 6 times the bed vol