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来源:百度知道 编辑:UC知道 时间:2024/06/22 19:37:02
L-tryptophan is a kind of necessary amino acid.It is useful for the growth of people and animals.There are several production methods of L-tryptophan,ultimately only L-tryptophan production by fermentation proved commercially feasible. Therefore,increasing the production and extraction of L-tryptophan are the problems that needed to be settled at present. Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the lar
L-tryptophan is a human and animal life in one of eight amino acids necessary for human and animal growth and development, play an important role in metabolism. L-Tryptophan has a variety of production methods, of which the most promising fermentation. Therefore, how to take effective measures to improve the fermentation broth of L-tryptophan production rate, and is refined out of the question of the immediate problem. In this paper, the selection of the kinds of resin, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research and exploration, and ultimately determine the strong acid cation resin HZ-001 at pH = 3,3 times the bed volume flow rate, temperature, room temperature, maximum of tryptophan adsorption; will Centrifugal fermentation broth to precipitate after decolorization with activated carbon through the HZ-001 strong acid cation exchange resin column, and then 2mol / L of ammonia flow rate of 6 times the bed vol