急求！！英语高手帮忙翻译下分子生物学的文章

来源：百度知道 编辑：UC知道 时间：2024/06/14 00:58:49
mRNA水平检测是从基因水平研究生理、病理反应的一项基本技术．实时荧光定量PCR(Real—time fluorescence quantitative polymerase chain reaction RFQ—PCR)是在PCR技术基础上建立的一种核酸定量技术，它具有操作简便、结果直观、敏感性高、特异性强、重复性好等优点，已成为基因检测和定量的重要方法 [5]．
本试验以寒冷刺激作为应激源，采用RFQ—PCR法，测定仔猪不同应激强度前后不同时间淋巴细胞中HSP70 mRNA的拷贝数，动态分析冷应激前后HSP70 mRNA转录量变化规律，从基因水平探讨不同应激强度对动物的影响及动物冷应激的反应机制，为评价HSP70 mRNA作为应激程度评估标准的可能性奠定基础．

the mRNA level examination is from the gene level research physiology, pathological reaction basic strokes. Real-time fluorescence quota PCR (Real-time fluorescence quantitative polymerase chain reaction RFQ-PCR) is one kind of nucleic acid quota technology which establishes in the PCR technology base, it has the operation to be simple, the result is direct-viewing, the sensitivity is high, the specificity is strong, duplication good and so on merits, has become the gene examination and quota important method [5]. this experiment takes the stress source by the cold stimulation, uses the RFQ-PCR law, around the determination newborn pig different stress intensity in the different time lymphocyte the HSP70 mRNA copy number, around the dynamic analysis cold stress HSP70 the mRNA duplication quantity change rule, from the gene level discussion different stress intensity to the animal the influence and the animal cold stress's response mechanism, to appraise HSP70 mRNA to lay the fou