悬赏20!!!急求论文医学论文摘要翻译~~!周二交(明天26日交)在线等
来源:百度知道 编辑:UC知道 时间:2024/06/04 03:57:59
关键词 冷冻保护剂;家猫;冷冻;精液
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Cats in order to improve the effectiveness of frozen semen, the test from the three conventional cryoprotectants (glycerol, dimethyl sulfoxide, ethylene glycol) in an optimal protection of screening agents, in different concentrations (3%, 5 %, 7%), different thawing temperature (30 ℃, 37 ℃, 50 ℃) under the frozen semen test, sperm motility after thawing, viability, acrosomal integrity and the rate of malformation rate of the four parameters as a test thaw sperm quality indicators to judge and choose a suitable domestic cat sperm cryopreservation method. The results showed that: the use of ethylene glycol cryoprotectant so protective agent,
Indicators of sperm after thawing were significantly higher than the glycerol group and DMSO group, and the concentration of 5% over 3% and 7% better concentration, thawing temperature is best at 37 ℃, its vitality can be 0.27 ± 0.08 (p <0.05), live rate 44.63 ± 5.24 (p <0.05), abnormal rate of 40.7 ± 3.3 (p <0.05), acrosome integri