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Self-designed primers, PCR method of application from the intestinal pathogenic E. coli (EPEC) chromosomal DNA was amplified the complete eae gene of the highly conserved reading frame of a size to be approximately 1800 bp fragment of the gene and sequenced. For the realization of lactic acid bacteria in E. coli and the eae gene expressed in conservative parts of the gastrointestinal tract using lactic acid bacteria to adapt to the environment, tolerance of the small intestine in the characteristics of a variety of digestive enzymes, intestinal adhesion and the advantages of strong conservative part of the eae gene of high homology Preparation can prevent E. coli diarrhea lead to a variety of infections, "food grade" lactic acid bacteria genetically engineered oral vaccine to explore the road.