求PCR(聚合酶链式反应)的英文介绍或综述

来源:百度知道 编辑:UC知道 时间:2024/06/20 05:03:47

给一篇免费英文综述链接,关于polymerase chain reaction的,希望能够对你有所帮助:
http://qjmed.oxfordjournals.org/cgi/reprint/78/3-4/195

Taq polymerase (pronounced; tack poll-im-er-aze) is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965[1]. It is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR), methods for greatly amplifying short segments of DNA.
T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified[1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR[2]. Therefore it replaced the DNA polymerase from E.coli originally used in PCR [3]. Taq's optimum temperature for activity is 75-80°C, with a h