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Method: Seeks method and appropriate in vitro growth condition whichis suitable, the good in vitro separation, raises and appraises persongallbladder epithelial cell. Then will test divides into two groups,the first group nanometer bacterium strain Se90 and person gallbladderepithelial cell will crawl the piece mix raise, the second group willcrawl the piece blank comparison group for gallbladder epithelialcell. Under the light mirror observes different density nanometerbacteriological attack posterity gallbladder epithelial cell themorphological change. After the use 四唑 salt (MTT) is attackedcompared to the color experiment examination the cell survival and thegrowth situation. The use immunity fluorescence dyes an observationdifferent time nanometer bacterium to the epithelial cell spaceposition relations. With flows the type cell meter examination thistime the cell weakly to perish the situation. In passes under theradio mirror to observe by the nanometer bacteriological attackga