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Pichia pastoris is a methylotrophic yeast that can be genetically engineered to express proteins for both basic research and industrial use [1晻]. Over 400 proteins, from human endostatin to spider dragline silk protein, have been produced in this yeast [2晻]. P. pastoris is uniquely suited for foreign protein expression for three key reasons: it can be easily manipulated at the molecular genetic level (e.g. gene targeting, high-frequency DNA transformation, cloning by functional complementation); it can express
proteins at high levels, intracellularly or extracellularly; and it can perform many ‘higher eukaryotic’ protein modifications, such as glycosylation, disulfide-bond formation, and proteolytic processing [3]. Because of these characteristics some proteins that cannot be expressed efficiently in bacteria, Saccharomyces cerevisiae or baculovirus have been successfully produced in functionally active form in P. pastoris [4晻]
Pichia pastoris is a methylotrophic yeast that can be genetically engineered to express proteins for both basic research and industrial use [1晻]. Over 400 proteins, from human endostatin to spider dragline silk protein, have been produced in this yeast [2晻]. P. pastoris is uniquely suited for foreign protein expression for three key reasons: it can be easily manipulated at the molecular genetic level (e.g. gene targeting, high-frequency DNA transformation, cloning by functional complementation); it can express
proteins at high levels, intracellularly or extracellularly; and it can perform many ‘higher eukaryotic’ protein modifications, such as glycosylation, disulfide-bond formation, and proteolytic processing [3]. Because of these characteristics some proteins that cannot be expressed efficiently in bacteria, Saccharomyces cerevisiae or baculovirus have been successfully produced in functionally active form in P. pastoris [4晻]
Pichia pastoris 是可能基因上被设计表达蛋白质至于基础研究和工业使用的methylotrophic 酵母[ 1? ] 。400 蛋白质, 由人的endostatin 对蜘蛛牵引索丝绸蛋白质, 被生产了在这酵母[ 2 里? ] 。P. pastoris 独特地适用与外国蛋白质表示为三个关键原因: 它可能容易地被操作在分子基因水平(即基因瞄准, 高频率DNA 变革, 克隆由功能complementation); 它可能表达 蛋白质在高水平, 细胞内或extracellularly; 并且它可能进行许多` 更高eukaryotic ' 蛋白质修改, 譬如glycosylation, 二硫化物结合形成, 和蛋白水解处理[ 3] 。由于这些特征无法用细菌、Saccharomyces Cerevisiae 或baculovirus 高效率地被表达的一些蛋白质成功地被生产了以功能上活跃形式在P. pastoris [ 4? ]