UC知道跪求高手猛人帮忙翻译！！！！急
来源：百度知道 编辑：UC知道 时间：2024/05/23 13:20:27
梨是全世界一个重要的，分布广泛的果树。栽培面积大，历史悠久。梨也是配子体自交不亲和的代表植物之一。S基因是影响植物自交不亲和的关键基因，现在世界上共发现了？个S等位基因，对植物自交不亲和机理的研究起着非常重要的作用。
本实验从？梨雌蕊中提取总RNA，然后运用RT-PCR，RACE等技术来获取S15基因的cDNA全长，并用琼脂糖凝胶电泳，核酸蛋白仪，套式PCE，质粒克隆，目的基因测序以及生物信息学软件来检测，分析提取RNA的质量。实验结果得到了高质量的RNA以及S15基因cDNA全长。
Pear is the world an important and widely scattered shrubs. Cultivation area, has a long history. Pear is gametophyte self-incompatibility and the representatives of one of the plants. S gene is affecting plant self-incompatibility and the key genes, the world discovered? S allele of plant self-incompatibility and mechanistic studies play a very important role. From this experiment? Pistil pears from the total RNA, then using RT-PCR, RACE S15 technology to obtain full-length cDNA, and with agarose gel electrophoresis, nucleic acid protein NG, Nested PCE, plasmid cloning, gene sequencing and bioinformatics software to detect, analyze the quality of RNA extraction. Experimental results of the quality of the RNA and S15 Full-length cDNA.
Pear pistil RNA withdraws and the effect analysis
The pear is world important, distributes the widespread fruittree. Cultivation area big, the history is glorious. The pear also is配子体 the inbred line does not kiss one of with represen