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来源：百度知道 编辑：UC知道 时间：2024/06/17 04:04:24
The global regulator FNR from Escherichia coli controls the transcription of
genes required for an anaerobic lifestyle. While previous studies have
demonstrated that FNR activity is regulated by O2 through loss of dimerization
upon destruction of its [4Fe-4S]2+ cluster, the present study reveals that
monomeric FNR protein is also a target of proteolysis. We have found that
turnover of FNR protein is increased selectively under aerobic growth
conditions, when FNR is not active as a transcription factor and is primarily a
metal-free, monomeric form (apo-FNR). This degradation of monomeric FNR was
dependent on the ClpXP protease and required the presence of two amino acid
sequences within FNR that resemble known ClpX recognition motifs. By measuring
the turnover rates of various FNR mutants that have unique properties with
respect to dimerization and Fe-S cluster stability, we have shown that loss of
dimerization upon [4Fe-4S]2+
genes required for an anaerobic lifestyle. While previous studies have
demonstrated that FNR activity is regulated by O2 through loss of dimerization
upon destruction of its [4Fe-4S]2+ cluster, the present study reveals that
monomeric FNR protein is also a target of proteolysis. We have found that
turnover of FNR protein is increased selectively under aerobic growth
conditions, when FNR is not active as a transcription factor and is primarily a
metal-free, monomeric form (apo-FNR). This degradation of monomeric FNR was
dependent on the ClpXP protease and required the presence of two amino acid
sequences within FNR that resemble known ClpX recognition motifs. By measuring
the turnover rates of various FNR mutants that have unique properties with
respect to dimerization and Fe-S cluster stability, we have shown that loss of
dimerization upon [4Fe-4S]2+
全球性管理者FNR 从大肠埃希氏菌控制副本 基因要求为一种绝氧生活方式。当早先研究有 显示出, FNR 活动由O2 调控通过二聚作用损失 在破坏它[ 4Fe-4S]2+ 群, 本研究显露那 monomeric FNR 蛋白质并且是解朊作用的目标。我们发现了那 FNR 蛋白质转交有选择性地被增加在有氧成长之下 情况, 当FNR 不是活跃的作为副本因素和是主要a 无金属, monomeric 形式(apo FNR) 。monomeric FNR 的这退化是 受抚养者在ClpXP 蛋白酶和要求出现二氨基酸 序列在类似已知的ClpX 认识主题的FNR 之内。由测量 有独特的物产各种各样的FNR 突变体的转交率 尊敬对二聚作用和Fe S 使稳定成群, 我们显示了那损失 二聚作用[ 4Fe-4S]2+ 群破坏由O2 瞄准FNR 为 退化由ClpXP 蛋白酶。另外, 由测量差别 FNR 退化的率在开关有氧文化对绝氧成长 情况, 我们提供证据, 已存在FNR apo-protein 可能是 转换对[ 4Fe-4S]2+ - FNR 。终于, 我们演讲生理意义 FNR 解朊作用由显示出, 变化的FNR 蛋白质成水平在小 范围在有氧成长情况下有直接效应在FNR 的作用 在O2 感觉。