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来源：百度知道编辑：UC知道时间：2024/05/27 07:06:27

imprinting of the
XIST locus in the bovine.
DNA methylation analysis of the bovine XIST
DMR. In exon 1 of the XIST locus, a CpG island was
detected at +1477 to +1683, as was determined by
CpG island prediction software (www.ebi.ac.uk/
emboss/cpgplot/) and a primer set was designed for
amplification of bisulfite-treated DNA encompassing
this area. Additionally, a (T/A) polymorphism
was detected at +1569 thereby allowing discrimination
between paternal and maternal X chromosomes
within this region. Liver and chorion samples from
an individual female were chosen since these tissues
had previously exhibited biallelic and monoallelic
expression, respectively, in all females analyzed.
Bisulfite sequencing of the CpG island, however,
proved to be difficult; it became apparent after analyzing
multiple sequences (>100), that possibly

印迹的
冷轨迹在牛。
甲基化分析了牛冷
移民和难民事务部。在第一外显子的冷轨迹，中央人民政府岛
发现在1477到1683 ，作为测定
cpg岛预测软件（ www.ebi.ac.uk/
热烫压花/ cpgplot / ）和底漆一套是专为
扩增的亚硫酸氢钠处理脱氧核糖核酸包罗万象
这方面的工作。此外，（吨/年）多态性
发现于1569从而使歧视
与父亲，母亲x染色体
在这一地区。肝脏和绒毛取样
个别女性选择了，因为这些组织
先前曾展出双等位基因及等位基因
表达，分别为，在所有女性的分析。
硫酸氢钠测序中央人民政府岛，但
被证明是困难的;很明显，经过分析
多序列（ > 100 ），这可能是
二级结构或高，在丰富的重复序列
已形成了从硫酸氢钠转化率和
抑制排序从过去出发，首
30 ¨ c50的基地，对笔录。修改后
单车参数，整整4全长序列
从肝脏样本，而不是从绒毛，
这表明互惠甲基中央人民政府
岛屿之间的父亲，母亲x色谱-
imprinting of the
XIST locus in the bovine.
DNA methylation analysis of the bovine XIST
DMR. In exon 1 of the XIST locus, a CpG island was
detected at +1477 to +1683, as was determined by
CpG island prediction software (www.ebi.ac.uk/
emboss/cpgplot/) and a

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