摘要翻译（中文—英文）

来源：百度知道编辑：UC知道时间：2024/06/17 07:21:34

摘要：以杨树叶片为外植体，进行组织培养试验研究。结果表明：叶脉和叶柄处极易产生不定芽；培养基中BA浓度是影响不定芽形成的关键；BA浓度在0.5～2mg/L 范围内均有不定芽产生，BA1.0 mg/L 与NAA0.05 mg/L 配合使用，可促进不定芽的形成；培养基中添加 GA3 0.1～0.2mg/L，可以提高不定芽的发生频率。在此基础上建立杨树叶片离体再生体系，利用该体系能够获得高的芽苗再生频率，但是不同品种间略有差异。

Abstract: poplar leaves explants, a tissue culture pilot study. The results show that: the vein and petiole easily produce adventitious buds; medium BA concentration is affected adventitious buds forming the key; BA concentration in the 0.5 to 2 mg / L of adventitious buds have returned BA1.0 mg / L and NAA0 .05 mg / L with use, can promote the formation of adventitious buds; medium add GA3 0.1 to 0.2 mg / L, can increase the frequency of adventitious buds. On this basis, the establishment of poplar leaves vitro regeneration system, the use of the system can be regenerated shoots high frequency, but a slight difference between the different varieties.

Abstract: Plants the body take the poplar tree leaf blade as outside,conducts the tissue culture experimental study. The result indicatedthat, The leaf vein and the petiole place is extremely easy to producedoes not decide the bud; In the culture medium the BA density is theinfluence does not decide the key which the bud forms; T

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