Affinity chromatography as a rapid and convenient method for purification of fungal laccases

Article
Affinity chromatography as a rapid and convenient method for purification of fungal laccases
J. Rogalski, M. Wojtas-Wasilewska, R. Apalovi, A. Leonowicz *
Department of Biochemistry, Maria Curie-Skodowska University, 20-031 Lublin, Poland

http://www3.interscience.wiley.com/cgi-bin/abstract/107622902/ABSTRACT?CRETRY=1&SRETRY=0
*Correspondence to A. Leonowicz, Department of Biochemistry, Maria Curie-Skodowska University, 20-031 Lublin, Poland

Keywords
laccase ?Trametes versicolor ?Fomes fomentarius ?affinity chromatography ?purification

Abstract
A rapid and convenient method for graduation, isolation, and purification of laccase from Trametes versicolor and Fomes fomentarius culture fluids was developed. For purification affinity chromatography on syringyl- and vanilly