帮忙翻译一段话 用英文

来源：百度知道 编辑：UC知道 时间：2024/05/21 08:48:06
绿色荧光蛋白（Green fluorescent protein， GFP），最早是在水母中发现的一种发光蛋白。本实验引用该基因并使其在大肠杆菌中进行表达、纯化和鉴定。具体操作为PCR扩增GFP基因，并将其插入到pTrcHis中，后将重组质粒转化到E.coli（BL21菌株）中，使GEP得以表达，运用Western Blot和SDS-PAGE证明了绿色荧光蛋白在E.coli中的表达。

Green fluorescent protein (Green fluorescent protein, GFP), was first found in a jellyfish luminescent protein. The experimental application of the gene and its expression in E. coli, purification and identification. PCR amplification of specific operations for the GFP gene and insert it into the pTrcHis, will be transformed into E.coli recombinant plasmid (BL21 strain), the GEP expressed, using Western Blot and SDS-PAGE proved that green fluorescent protein in E.coli.

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The green fluorescence protein (Green fluorescent protein, GFP), is one kind of luminescent protein which most early discovered in the jellyfish. This experiment quotes this gene and causes it to carry on the expression, the purification and the appraisal in the backwoods coli. The concrete operations are PCR increase the GFP gene, and inserts it to pTrcHis, latter will reorganize the material particle to transform to E.coli (the BL21 strain), enables GEP to express, has proven the green fluorescence p