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2. Materials and methods
2.1. Experimental setup
A first experiment on biological treatment of cyanide
(experiment no. 1) was carried out in seven reactors, each of
them consisting of a highly mixed aerated tank connected to
a clarifier. Each reactor was equipped with two peristaltic
pumps, one for feeding synthetic wastewater and one for
recycling sludge from the clarifier. Reactor no. 1 had a 3-L
aeration tank volume and reactor nos. 2–7 had a 2-L volume.
Clarification systems were stirred and designed to ensure
recycled sludge retention times smaller than 1/2 h.
The mixing of aeration tanks content was achieved by
mechanical stirring. With a stirring power higher than
3WL1, reactor hydraulics was considered to be close to
perfect mixing, and effluent concentrations were considered
to represent the concentration of aeration tanks. DO in the
reactor no. 1 was regulated at 3mg L1 and the others reactor

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