UC知道专业英语翻译，急寻高手哦
来源：百度知道 编辑：UC知道 时间：2024/05/20 16:09:42
3.5. Short fiber forming activity
Release of significant amount of short fibers as well as reducing
groups from filter paper due to enzymatic action was
observed on prolonged incubation (10 h) of reaction mixture.
The release of short fibers can be observed within 15–30 min.
The fragmentation of filter paper increased initially with
increase in time. However, there is decrease in short fiber
formation after 4 h of interval without decrease in reducing
sugars formation. This may be attributed to preferential
hydrolysis of short fibers formed by endoglucanase components.
Both Endo A and Endo B showed similar patterns of
short fiber formation. The Endo B fraction with relatively
low molecular weight than Endo A, showed more short fiber
forming activity. Halliwell and Riaz [21] demonstrated that
the low molecular weight endoglucanase from T. koningii
is responsible for the formation of short fibers from native
Release of significant amount of short fibers as well as reducing
groups from filter paper due to enzymatic action was
observed on prolonged incubation (10 h) of reaction mixture.
The release of short fibers can be observed within 15–30 min.
The fragmentation of filter paper increased initially with
increase in time. However, there is decrease in short fiber
formation after 4 h of interval without decrease in reducing
sugars formation. This may be attributed to preferential
hydrolysis of short fibers formed by endoglucanase components.
Both Endo A and Endo B showed similar patterns of
short fiber formation. The Endo B fraction with relatively
low molecular weight than Endo A, showed more short fiber
forming activity. Halliwell and Riaz [21] demonstrated that
the low molecular weight endoglucanase from T. koningii
is responsible for the formation of short fibers from native
3.5 。短纤维的形成活动
释放大量的短纤维，以及减少
集团从滤纸，由于酶法行动
就长期观察孵化（ 10 h ）的反应混合物。
释放短纤维可以观察15-30分钟。
分散的滤纸增加，最初与
增加的时间。不过，有减少，短纤维
形成4 h后的区间，没有减少，在减少
糖的形成。这可能是由于优惠
水解短纤维所形成的内切组件。
双方远藤一和远藤b表明，类似的模式
短纤维的形成。该远藤b分数与相对
低分子量比远藤一，显示更多的短纤维
形成的活动。利维尔和里亚兹[ 21 ]证明
低分子量内切从汤匙， koningii
负责形成短纤维，从本土
纤维素衬底。 1,4 - _ - D -葡聚糖cellobiohydrolase
从汤匙，绿色纯化berghem和petersson [ 31,32 ]
是缺乏短纤维形成的活动。然而， 2
纯化endoglucanases从相同的文化表明，短期
纤维形成的活动。与此相反，远藤类型的模式
行动1,4 - _ - D -葡聚糖cellobiohydrolase由S. rolfsii
表现sadana和帕蒂尔[ 33 ] 。扫描
电子显微镜研究证实，短纤维的形成
由远藤乙的平均规模短纤维后形成的
6 h后孵化与远藤b不同之间的20和100 _m
相比300和500 _m与控制
酶。
3.6 。吸附特性的纤维素酶对
不溶性基质
为不溶性基质系统，初始速率水解
是不成正比的总金额酶
在该系统。当影响酶浓度对
率水解滤纸， mow纸浆， avicel和
蔗渣浆进行了研究数据符合方程
开发麦克拉伦[ 22 ]描述水解率
酶催化反应在异构系统（图5 ） 。
那个
n值为滤纸， mow纸浆， avicel和蔗渣
髓计算应为0.31 ， 0.44 ， 0.28 ， 0.50 ，分别。
可比n值也观察到在案件
胞外纤维素酶