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摘要 目的: 通过用紫外分光光度法对不同采收期半枝莲总黄酮含量的测定,了解不同采收期药材总黄酮含量的差别,为半枝莲合理开发利用、优质高产以及制定科学的质量标准提供理论依据。方法:采用紫外分光光度法对不同采收期半枝莲中野黄芩苷含量进行测定。结果:5月26日、7月28日、9月23日三个不同采收期半枝莲中总黄酮含量分别为2.20%、2.00%、1.72%。野黄芩苷的线性范围为r=0.9993,平均加样回收率为RSD为100.79% 结论:紫外分光光度法简便、准确、可靠而且不同采收期对半枝莲总黄酮的百分含量影响较大,可作为半枝莲药材内在质量控制手段之一。
关键词: 半枝莲;紫外分光光度法;不同采收期;总黄酮;含量测定
图1-4半枝莲茎粉末显微图
图1-5半枝莲叶粉末显微图

图1-6半枝莲果实（带宿萼）粉末显微图
Fig.1-6
表1 半枝莲物候期（单位日／月）
图2-1野黄芩苷对照品溶液紫外吸收光谱图
Fig.2-1
图2-2半枝莲样品溶液紫外吸收光谱图
Fig.2-2
表2 半枝莲总黄酮测定重复性试验结果(n=6)
表3 半枝莲总黄酮含量测定标准曲线
图2-3 半枝莲总黄酮含量测定标准曲线图
Fig.2-3
表4 半枝莲总黄酮含量测定精密度试验结果（n=6）
表5 半枝莲总黄酮含量测定加样回收率试验结果（n=6）
表6 不同采收期对半枝莲总黄酮含量测定结果

Abstract Objective: To use UV spectrophotometry of different harvesting period Scutellaria barbata Determination of Flavonoids in, understand the different herbs harvesting period of flavonoids in the difference, for the rational development and use of Scutellaria barbata, and the development of high quality and high yield The scientific quality standards and provide a theoretical basis. Method: UV spectrophotometry Scutellaria barbata different harvest time in the wild baicalin content was measured. Results: May 26, July 28, September 23 three different harvesting period of Flavonoids in Scutellaria barbata were 2.20 percent, 2.00 percent, 1.72 percent. Ueno baicalin in the range of r = 0.9993, average recovery for the RSD was 100.79% Conclusion: UV spectrophotometry simple, accurate, reliable and different harvest time on the percentage of flavonoids Scutellaria barbata A greater impact on the content, Scutellaria barbata herbs can be used as means of internal quality control.