医学英文翻译

来源：百度知道编辑：UC知道时间：2024/05/26 07:18:48

用抗大鼠COX-2单抗包被于酶标板上，标准品和样品中的COX-2与单抗结合，加入生物素化的抗大鼠COX-2,形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在450nm处测OD值，COX-2浓度与OD值成正比，以标准品 20，10，5，2.5,1.25,0.625,0.312, 0 ng/ml为横坐标，OD值为纵坐标，画出标准曲线, 查出相应COX-2含量.（翻译成英文）急

Coated ELISA plates with COX-2 anti-rat monoclonal antibody,then add biotinylated anti-rat COX-2 after the standard and samples of COX-2 combined with the monoclonal antibody.And the Streptavidin which marked by Horse radish peroxide enzyme combined with biotin. Then joins substrate driving fluid obviously blue color.Finally, add the termination of liquid sulfuric acid,measure the value of OD at 450nm,then draw the standard curve in a coordinate system which useing
standard 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value for longitudinal coordinates.
And then, identify the corresponding COX-2 content.

With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liqui