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来源:百度知道 编辑:UC知道 时间:2024/06/08 11:56:53
大别山土壤放线菌活性菌株的筛选及鉴定
摘要:本研究通过对分离自大别山的土壤放线菌的筛选,得到活性菌株,通过形态观察及16SrDNA的测定,初步确定活性菌株的分类地位,为进一步研究积累参考数据。对30株采自大别山的菌种进行抑菌实验,分别利用真菌和细菌作为指示菌,来检测菌种的生物活性。对有生物活性的菌株进行进一步的生物学特征检测,对高活性菌株测定16SrDNA。首先活化菌种,将菌种制成发酵液,活化4种指示菌,接发酵液观察其抑菌性。根据初筛结果选取高活性的菌株复筛,将初筛的发酵液再次过滤,实验过程大体同初筛,活性菌株的形态观察 通过插片实验观察其生物学特征,16SrDNA的测定。最后通过对30株放线菌的初步筛选和再次筛选,找到有生物活性的菌株,通过对活性菌株的16SrDNA的测定和生物学特征的检测,初步确定其分类地位。
关键词:土壤;放线菌;活性;筛选;鉴定

Dabieshan activity of soil actinomycetes screening and identification of strains
Abstract: In this study, isolated from the Dabie Mountains of soil actinomycetes screening, has been active strains 16SrDNA through morphological observation and the determination of the initial activity to determine the taxonomic status of strains, the accumulation of reference data for further study. Of 30 strains collected from the Dabie Mountains to the inhibitory experiments, the use of fungi and bacteria, respectively, as indicator bacteria, to detect biological activity of bacteria. To have the biological activity of strains of biological characteristics for further testing, the determination of high-activity strains 16SrDNA. Activation of bacteria First of all, will be made from the fermentation culture fluid, activation of four kinds of bacteria instructions, and then observe the inhibitory fermentation broth of. According to the results of screening to select strains with high activity s