UC知道帮忙翻译一下3～
来源：百度知道 编辑：UC知道 时间：2024/06/08 13:45:51
A sickly mutant
The key to the eventual discovery of activase was theArabidopsis rcamutant, which was reported by Somerville et al. (1982). This mutant came through the high CO2/low CO2screen developed by Chris Somerville, while a postdoc in Bill Ogren’s USDA laboratory located in the Agronomy Department in Turner Hall at the University of Illinois (Figure 1). The screen was designed to isolate mutants with defects in the photorespiratory pathway, a radical approach at the time for resolving controversies about metabolic pathways in plants. Mutant CS207 was just one of many that passed the initial screen, but initially it did not grow very well even under high CO2and could easily have been discarded. Fortunately, Chris was meticulous and he took the time to backcross such plants to the wild-type in order to eliminate spurious mutations.
Chris Somerville’s usual approach for characterizing these mutants was to label them with14C and then apply a simple batch ionic
The key to the eventual discovery of activase was theArabidopsis rcamutant, which was reported by Somerville et al. (1982). This mutant came through the high CO2/low CO2screen developed by Chris Somerville, while a postdoc in Bill Ogren’s USDA laboratory located in the Agronomy Department in Turner Hall at the University of Illinois (Figure 1). The screen was designed to isolate mutants with defects in the photorespiratory pathway, a radical approach at the time for resolving controversies about metabolic pathways in plants. Mutant CS207 was just one of many that passed the initial screen, but initially it did not grow very well even under high CO2and could easily have been discarded. Fortunately, Chris was meticulous and he took the time to backcross such plants to the wild-type in order to eliminate spurious mutations.
Chris Somerville’s usual approach for characterizing these mutants was to label them with14C and then apply a simple batch ionic
甲病突变的关键，最终发现是theArabidopsis rcamutant活，这是报告的萨默维尔等。 （ 1982年） 。这种突变是通过高级CO2/low CO2screen开发的克里斯默维尔，而博士后在比尔更胜一筹的美国农业部实验室位于农学系部特纳大厅伊利诺斯大学（图1 ） 。屏幕的目的是孤立的突变体与缺陷光呼吸途径，一个激进的办法在解决争议的时间大约在植物代谢途径。突变体CS207是一个许多人通过了最初的屏幕，但最初它的增长速度也没有很好，即使在高CO2and可以很容易地被取消。幸运的是，克里斯是一丝不苟，他花时间向回交这种植物的野生型，以消除杂散突变。
克里斯萨默维尔的惯常做法的特点是，这些突变体标签他们with14C然后套用一个简单的批处理离子交换分离，以确定潜在的病变的化合物的积累。突变体CS207很有意思，因为在低CO2it积累的标签在强酸，通常卡尔文-班森周期中间，而不是弱者酸或基地，预计在与突变体光呼吸途径。阿尔奇Portis ，新来港定居在厄巴本人和设在各地的实验室直接从比尔更胜一筹的，成立了一个离子交换高压液相色谱法（ HPLC ）系统专用？ cally解决卡尔文-班森周期中间体。克里斯来到他一天的一些样品，并询问他是否会为他色谱他们。他们惊讶地看到，大量标记的RuBP池，表明Rubisco活性的问题。然而，当克里斯分离蛋白的Rubisco活化酶的突变体，并检测它， Rubisco活化酶似乎完全正常。