关于荧光标记的，翻译成英文

来源：百度知道 编辑：UC知道 时间：2024/06/18 06:55:33
异硫氰酸荧光素 (fluorescein isothiocyanate, FITC)纯品为黄色或橙黄色结晶粉末，易溶于水和酒精溶剂。有两种异构体，其中异构体Ⅰ型在效率、稳定性与蛋白质结合力等方面都更优良。FITC分子量为389.4，最大吸收光波长为490～495nm，最大发射光波长为520～530nm，呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年，是目前应用最广泛的荧光素。其主要优点是人眼对黄绿色较为敏感，通常切片标本中的绿色荧光少于红色。用FITC标记的各种抗体]，多肽，多糖]等物质已广泛用于各种生物研究。

FITC有两种同分异构体，其中异构体A在效率、稳定性、与蛋白质结合能力等方面都更好，在冷暗，干燥处可保存多年，另外由于人眼对黄绿色较为敏感，通常切片标本中的绿色荧光少于红色，所以FITC成为使用最广泛的荧光素。用FITC标记的各种抗体，多肽，多糖等物质已广泛用于各种生物研究。
近年来对于药物载体的研究，也常使用荧光物质。将荧光物质引入药物载体的方法有：（1）通过化学结合的方法使荧光物质与聚合物耦联，再将耦联后的聚合物制备成载药微粒或者直接将荧光物质耦联在制备好的微粒表面。（2）直接将荧光物质或标记的药物包载进纳米颗粒。
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For the yellow or orange FITC tasted crystalline powder, soluble in water and alcohol solvent. Have two kinds of optical isomers, Ⅰ type in efficiency, stability and protein are more excellent adhesion, etc. For 389.4 FITC molecular weight, the maximum absorption wavelength for 490-495nm, maximum emission wavelength for 520-530nm, presents the bright green fluorescence. In the cold dark FITC dry can save years, is the most widely used fluorescent element. Its main advantage is sensitive to yellow eye, usually less than the green fluorescence sliced red specimens. With FITC markers of antibody], peptides, polysaccharide substances such as] has been widely used in various biological research.

FITC have two points, including A in optical isomers efficiency, stability, and protein better ability, etc, in cold dark, dry place for many years, can save another sensitive to yellow-green due to human, usually less than the green fluorescence sliced specimen FITC become red, so the