急求翻译5~

来源：百度知道 编辑：UC知道 时间：2024/06/25 21:07:37
3. Results and discussion
3.1. Method development
In order to propose a specific and accurate CE method for analyzing pharmaceutical products containing neomycin sulfate and polymyxin B sulfate, it is essential to find the best experi-mental conditions in which the analytes can be separated from each other. Because higher separation numbers can be obtained at low apparent mobilities, experiments were carried out in the anionic mode (anode at the detection side) with a reversed EOF. Since,neomycinsulfateandpolymyxinBsulfateformpositively charge ions in low pH buffer. One of the undesirable problems encountered in CE separation is adsorption onto the capillary wall, which can cause peak tailing and poor resolution. In this experiment, TTAB was added to the BGE in order to reverse the direction of EOF.
3.1.1. Selection of chromophoric ion
The correct choice of a chromophoric ion suitable for indi-rect detection that can form part of a suitably buffered

3。结果与讨论
3.1%。开发方法,
为了提出specific和精确的CE认证的方法,对医药产品含有硫酸和多粘菌素硫酸潜在威胁,很有必要find experi-mental最好的样本可以互相分离。由于可以获得更高的分离的数字低表观机动性,进行试验的阴离子模式(阳极的一面)检测反EOF。neomycinsulfateandpolymyxinBsulfateformpositively电荷离子,低pH缓冲。不良的问题之一是遇到CE分离上的吸附性能的毛细血管壁,这可能会导致尾矿和可怜的决议案。峰值在这个实验中,TTAB被添加到苷为反方向的EOF。
3.1.1。选择的chromophoric离子
正确的选择chromophoric离子适合indi-rect检测,可以构成一个适当的缓冲elec-trolyte不是完成简单明了。除了作为chromophoric离子,该化合物和家应该也有类似的电泳机动性。事实上,当elec-trophoretic机动性,significant峰tail-ing是不可能被观察到。或者让在初步工作,chromophoric离子biphenylamine以及胞嘧啶、N -(4-hydroxyphenyl)acetamide试验,finally N -(4-hydroxyphenyl)acetamide被选中,因为最好的峰形曲线。样本为了提高方法sen-sitivity、磨牙的吸收率的chromophoric离子应高及其吸收波长和ofanalytes不能重叠。图2 showstheabsorptionprofilesofneomycinsul-fate、多粘菌素硫酸根和N -(4-hydroxyphenyl acetamide)。N -(4-Hydroxyphenyl)acetamide吸收强烈的vicin-ity 2450海里有很大的吸收率磨牙。因为可用的280-nmfilter是一种用于硫酸潜在手段,也并行工程和多粘菌素硫酸根有mini-mum absorbances在这波长。一个探测280海里被选定为一个合适的妥协的chromophoric absorp-tion强离子和最小的吸收的样本。