硫酸铵沉降法纯化蛋白质 怎样进一步提高其纯化倍数

First of all, when you use ammonium sulfate (AS) to precipitate the proteins, you can use different concentrations of AS. For instance, you can begin with 30%, then, 40%, 50%, 60%, 80%. You can run a gel (or Western blot, or other approaches, such as activity assay) to see which fraction your protein is in. After dialysis against desired buffer you could do the further purification, these include chromatography. After those you can obtain pure protein.

Good luck.

2D电泳 效果很好